Description
Aviva Systems Biology LDHB ELISA Kit (Human) (OKEH03476) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A mouse monoclonal antibody specific for LDHB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated.After washing,abiotinylated detector antibody specific for LDHB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LDHB captured in well